Last data update: May 13, 2024. (Total: 46773 publications since 2009)
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Query Trace: Monteneri JA[original query] |
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Biofilm formation is not required for early-phase transmission of Yersinia pestis
Vetter SM , Eisen RJ , Schotthoefer AM , Monteneri JA , Holmes JL , Bobrov AG , Bearden SW , Perry RD , Gage KL . Microbiology (Reading) 2010 156 2216-2225 Our study investigated whether hms-mediated biofilm formation was necessary for early-phase transmission (EPT) of Yersinia pestis. In addition to the biofilm-dependent blockage model of plague transmission from flea to mammal, an EPT model of transmission, has been described where fleas transmit Y. pestis to a host up to 4 days post infection, which is insufficient time for blockages to form in flea foreguts. An artificial feeding system was used to feed Xenopsylla cheopis and Oropsylla montana rat blood spiked with either the parental Y. pestis strain KIM5(pCD1)+, two different biofilm-mutants (DeltahmsT , DeltahmsR), or a biofilm-overproducer mutant (DeltahmsP ). Infected fleas were then allowed to feed on naive Swiss Webster mice 1-4 days after infection and the mice were monitored for signs of infection up to three weeks post-exposure. The biofilm-defective mutants were transmitted from X. cheopis and O. montana as efficiently as the parent strain, whereas the transmission efficiency of fleas fed the biofilm-overproducer was significantly less than either the parent or biofilm-deficient strains. The bacterial loads in fleas infected with a biofilm-deficient strain harbored lower bacterial loads 4 days post infection when compared to fleas infected with the parent strain. Thus defects in biofilm formation did not prevent flea-borne transmission of Y. pestis in our EPT model and biofilm over-production inhibited efficient EPT, however biofilm may play a role in infection persistence in the flea. |
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